Kunová, Simona and Ivanišová, Eva and Žiarovská, Jana and Zamiešková, Lucia and Felsöciová, Soňa and Trajkovska-Petkoska, Anka and Nikolovska Nedelkoska, Daniela and Kačániová, Miroslava (2020) Differences between microbiota, phytochemical, antioxidant profile and DNA fingerprinting of Cabernet Sauvignon grape from Slovakia and Macedonia. Potravinarstvo Slovak Journal of Food Sciences.
Full text not available from this repository.Abstract
This study aimedto evaluate the microbiota, phytochemical, antioxidant profileand DNA fingerprinting of Cabernet Sauvignon grapesfrom Slovakia and R. NorthMacedonia. There were used two samples of grape berries (one sample from Slovakia and one from Macedonia). Each sample was analyzed in triplicate. The bacteria were cultivated on Plate countagar (PCA), microscopic filamentous fungi were cultivated on Malt extract agar (MEA). MALDI-TOF MS Biotyper mass spectrometrywas used for the identification of microorganisms (bacteria and yeasts) and microscopic filamentous fungi with manuals.DPPH methodwas used to determine of antioxidant activity of grape berries. Phytochemical and antioxidant profiles wereevaluated in grape berries samples. Total genomic DNA was extracted from mature grapes by GeneJET Plant Genomic DNA Purification Kit. The number of bacteria was higher in the sample of Macedonian grape (4.13 log CFU.g-1) in comparison to the grape from Slovakiaas well as the number of yeasts was alsohigher in the Macedonian sample (2.57 log CFU.g-1). Antioxidant activity of Slovak grape berries was 0.55 mg TEAC.g-1and of Macedonian grape,berries was 0.51 mg TEAC.g-1. Total polyphenol content was higher in grape from Slovakia (0.81 mg GAE.g-1) than in grape from Macedonia (0.77 mg GAE.g-1), while total flavonoid content was 0.57 and 0.17 mg QE.g-1in Slovak grape and Macedonian grape, respectively. Total phenolic acidcontent was higher in the sample from Macedonia (0.40mg CAE.g-1) compared to the grape from Slovakia (0.24 mg CAE.g-1). Total anthocyanin content was also higher in Macedonian grape (0.46 mg.g-1) comparedto the Slovak sample (0.05 mg.g-1). The total polymorphism for all of the used primers of 87.5% was obtained for the Macedonian sample of CabernetSauvignon and 89.4% for the Slovak sample.
Keywords: grape berries; bacteria; yeasts; antioxidant profile; MALDI-TOF MS Biotyper; polymorphism
| Item Type: | Article |
|---|---|
| Subjects: | Scientific Fields (Frascati) > Agricultural Sciences > Other agricultural sciences |
| Divisions: | Faculty of Technology and Technical Sciences |
| Depositing User: | Mr Jordan Martinovski |
| Date Deposited: | 29 Mar 2024 10:24 |
| Last Modified: | 29 Mar 2024 10:24 |
| URI: | https://eprints.uklo.edu.mk/id/eprint/9886 |
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