IMPACT OF MAMMARY GLAND INFECTIVE STATUS ON THE CONCENTRATION OF LACTOFERRIN IN COW’S MILK

Trajkovska, Biljana and Kochoski, Ljupche and Petrovski, Kiro and Dimitrovska, Gordana and Hajrulai-Musliu, Zehra and Uzunov, Risto and Petkov, Vladimir (2021) IMPACT OF MAMMARY GLAND INFECTIVE STATUS ON THE CONCENTRATION OF LACTOFERRIN IN COW’S MILK. Journal of Hygienic Engineering and Design, 36. pp. 91-95. ISSN 1857- 8489

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Abstract

Lactoferrin (LF) is glycoprotein, which is normally excreted in cow’s milk, but as a result of inflammatory processes in the mammary gland, its concentration is being increased. The aim of this research was to determinate whether the LF concentration in milk is influenced by increased number of milk somatic cell count. In this research we used milk from (n = 345) Holstein- Friesian cows in Pelagonia region, or more precisely milk from healthy (n = 112) and infected quarters (n= 233). A quarter was classified as healthy when no visible signs of mastitis were detected or the California Mastitis Test (CMT) was negative. A quarter was classified as subclinical when no visible signs of mastitis were detected but CMT was positive. A quarter was classified as clinical mastitis when there were visible signs of mastitis (cow, udder or milk appearance). The milk samples were analysed instrumentally for physico- chemical properties (Lactoscope C4+), somatic cell count (SCC) by Bentley Somacount CC 150, total number of bacteria (CFU) (by Bentley Bactocount IBC), and by ELISA for concentrations of lactoferrin. We used SPSS 15.0 for analysis of gained data. Significant differences were detected in concentrations of lactoferrin in healthy quarters (915.94 μg/mL) and in quarters with sub-clinical (2,095.87 μg/mL) and clinical mastitis (3,528.99 μg/mL) (p < 0.001). A correlation between lactoferrin and lactose (r = -0.21, p < 0.01), lactoferrin and CFU (r = 0.28, p < 0.01) as well as between lactoferrin and somatic cells (r = 0.32, p < 0.01) was observed. These results initiate that lactoferrin could be used as a complementary test to somatic cell count, and potential to be a primary diagnostic test for subclinical and clinical mastitis in dairy cows. Furthermore, it could be used as a natural antibiotic in treatment of mastitis in dairy cows, in order to decrease the presence of antibiotic residuals in milk, thereby improving the hygiene of raw milk.

Item Type: Article
Uncontrolled Keywords: Lactoferrin, Cow’s milk, Somatic cells, Mastitis
Subjects: Scientific Fields (Frascati) > Agricultural Sciences > Animal and diary science
Scientific Fields (Frascati) > Agricultural Sciences > Agriculture, forestry and fisheries
Divisions: Faculty of Biotechnical Sciences
Depositing User: Prof. d-r Biljana Trajkovska
Date Deposited: 04 Feb 2022 08:16
Last Modified: 04 Feb 2022 08:16
URI: http://eprints.uklo.edu.mk/id/eprint/6764

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