Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria

Dimitrova, Lyudmila and Kaleva, Mila and Zaharieva, Maya and Stoykova, Christina and Tsvetkova, Iva and Angelovska, Maja and Ilieva, Yana and Kussovski, Vesselin and Naydenska, Sevda and Najdenski, Hristo (2021) Prevalence of Antibiotic-Resistant Escherichia coli Isolated from Swine Faeces and Lagoons in Bulgaria. Antibiotics.

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Abstract

Abstract: Antimicrobial resistance (AMR) is a worldwide health problem affecting humans, animals,
and the environment within the framework of the “One Health” concept. The aim of our study
was to evaluate the prevalence of pathogenic strains of the species Escherichia coli (E. coli), their
AMR profile, and biofilm-forming potential. The isolated strains from three swine faeces and free
lagoons (ISO 16654:2001/Amd 1:2017) were confirmed using Phoenix M50 and 16S rDNA PCR. The
antibiotic sensitivity to 34 clinically applied antibiotics was determined by Phoenix M50 and the disc
diffusion method, according to the protocols of the CLSI and EUCAST. We confirmed the presence of
16 E. coli isolates, of which 87.5% were multi-drug-resistant and 31.25% performed strong biofilms.
The possibility for the carrying and transmission of antibiotic-resistance genes to quinolones (qnr),
aminoglycosides (aac(3)), β-lactamase-producing plasmid genes ampC, and blaSHV/blaTEM was
investigated. We confirmed the carrying of blaSHV/blaTEM in one and ampC in seven isolates. The
strains were negative for the virulence genes (ETEC (LT, STa, and F4), EPEC (eae), and STEC/VTEC
(stx and stx2all)). The results should contribute to the development of effective measures for limitation
and control on the use of antibiotics, which is a key point in the WHO action plan.

Item Type:	Article
Subjects:	Scientific Fields (Frascati) > Agricultural Sciences > Veterinary science
Divisions:	Faculty of Veterinary Medicine
Depositing User:	Mr Aleksandar Lozanovski
Date Deposited:	25 Mar 2024 10:06
Last Modified:	25 Mar 2024 10:06
URI:	https://eprints.uklo.edu.mk/id/eprint/9867

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