METHOD OPTIMIZATION FOR ISOLATION AND RECRYSTALLIZATION OF COMMERCIAL CURCUMIN EXTRACT: TLC AND COLUMN CHROMATOGRAPHY

Lozanovski, Zlatko and Nestorovska, Tatjana and Jankulovska, Angelka and Petkovska, Daniela and Arsovski, Denis (2024) METHOD OPTIMIZATION FOR ISOLATION AND RECRYSTALLIZATION OF COMMERCIAL CURCUMIN EXTRACT: TLC AND COLUMN CHROMATOGRAPHY. International Journal Knowledge, 64 (3). pp. 321-327. ISSN 2545-4439

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Abstract

Curcumin is an active component of the rhizome perennial plant turmeric (Curcuma longa) which belongs to the ginger family (fam. Zingiberaceae). Depending on the origin and the country where it grows, turmeric contains from 2% to 6% curcuminoids. The dominant component is curcumin (CUR), followed by demotoxicurcumin (DMCUR) and bisdemotoxicurcumin (BDMCUR). In the literature there is no simple reproducible method for obtaining curcumin on a microscale, therefore we optimized a procedure for the purification of curcumin from a mixture with other curcuminoids. The best method of purification is by a cross-precipitation combination of 10:1 (V/V) 2-propanol/acetone, finalizedby gradient-elutioncolumn chromatography 99:1 (V/V) dichloromethane/methanol, 97:3 (V/V) dichloromethane/methanol and 95:5 (V/V) dichloromethane/methanol. The purity of the products can be checked by TLC and the key is the choice of a solvent that should move the products with an Rfvalue above 0.3. For this purpose, 97:3 (V/V) dichloromethane/methanol was most suitable. Although spots on silica gel plates can be seen with the eye, they are best visualized with a UV lamp at a wavelength of 254 nm or 366 nm.

Item Type:	Article
Subjects:	Scientific Fields (Frascati) > Natural sciences > Chemical sciences Scientific Fields (Frascati) > Medical and Health Sciences > Other medical sciences
Divisions:	Higher Medical School
Depositing User:	MSc Denis Arsovski
Date Deposited:	03 Sep 2024 08:44
Last Modified:	01 Oct 2024 11:57
URI:	https://eprints.uklo.edu.mk/id/eprint/10055

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