HPLC method for determination of some psychostimulative drugs using derivatization reagent

Pavlova, Valentina and Menkinoska, Marija and Blazevska, Tatjana and Gjorgovska, N. (2014) HPLC method for determination of some psychostimulative drugs using derivatization reagent. Horizons-series B. pp. 173-182. ISSN 1857-8578

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Abstract

Different alternatives for the rapid and sensitive determination of some psychostimulative drugs (MDA, MDMA, MDEA, MBDB), by Highperformance liquid chromatography (HPLC) have been evaluated. The method involved derivatization with 3,5-dinitrobenzoylchloride at ambient temperature and separation of the derivatives formed on a Lichrospher® 60 RP-select B, 5 µm, 250 x 4,6 mm id column protected by appropriate guard column. Conditions for the derivatization have been investigated, including the pH, the reaction time and the derivatization reagent concentration. After optimisation, the best results obtained using buffer with pH 10.0, reaction time 5 min and concentration of derivatization reagent 5 mmol/L. Key words: determination, psychostimulative drugs, derivatization, Highperformance liquid chromatography

Item Type: Article
Subjects: Scientific Fields (Frascati) > Natural sciences > Chemical sciences
Divisions: Faculty of Technology and Technical Sciences
Depositing User: Mr Jordan Martinovski
Date Deposited: 06 Feb 2019 16:43
Last Modified: 07 Aug 2019 09:30
URI: http://eprints.uklo.edu.mk/id/eprint/1663

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