Prevalence, Genetic Homogeneity, and Antibiotic Resistance of Pathogenic Yersinia enterocolitica Strains Isolated from Slaughtered Pigs in Bulgaria

Angelovska, Maja and Zaharieva, Maya and Dimitrova, Lyudmila and Dimova, Tanya and Gotova, Irina and Urshev, Zoltan and Ilieva, Yana and Kaleva, Mila and Chan Kim, Tanya and Naydenska, Sevda and Dimitrov, Zvechko and Najdenski, Hristo (2023) Prevalence, Genetic Homogeneity, and Antibiotic Resistance of Pathogenic Yersinia enterocolitica Strains Isolated from Slaughtered Pigs in Bulgaria. Antibiotics (12).

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Abstract

Abstract: Yersiniosis is the third most commonly reported foodborne zoonosis in the European
Union. Here, we evaluated the prevalence of pathogenic Yersinia enterocolitica among healthy pigs
(as a major reservoir) in a slaughterhouse in Bulgaria. A total of 790 tonsils and feces from 601 pigs
were examined. Isolation and pathogenicity characterization was carried out by the ISO 10273:2003
protocol and Polymerase Chain Reaction (PCR), detecting the 16S rRNA gene, attachment and
invasion locus (ail), Yersinia heat-stable enterotoxin (ystA), and Yersinia adhesion (yadA) genes. Genetic
diversity was assessed by pulsed-field gel electrophoresis (PFGE), and antimicrobial resistance by
the standard disk diffusion method. Of all the pigs tested, 6.7% were positive for Y. enterocolitica.
All isolates belonged to Y. enterocolitica bioserotype 4/O:3. ail, and ystA genes were detected in all
positive strains (n = 43), while the plasmid Yersinia virulence plasmid (pYV) was detected in 41. High
homogeneity was observed among the strains, with all strains susceptible to ceftriaxone, amikacin
and ciprofloxacin, and resistant to ampicillin. In conclusion, a low prevalence of Y. enterocolitica 4/O:3
was found in healthy pigs slaughtered in Bulgaria, not underestimating possible contamination of
pork as a potential risk to consumer health.
Keywords: pathogenic Yersinia enterocolitica; pigs; PCR; virulence genes; antibiotic resistance;
pulsed-field gel electrophoresis

Item Type:	Article
Subjects:	Scientific Fields (Frascati) > Agricultural Sciences > Veterinary science
Divisions:	Faculty of Veterinary Medicine
Depositing User:	Mr Aleksandar Lozanovski
Date Deposited:	25 Mar 2024 10:05
Last Modified:	25 Mar 2024 10:05
URI:	https://eprints.uklo.edu.mk/id/eprint/9865

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