Bacteria variety causing clinical mastitis in Holstein-Friesian cows in Pelagonia region, North Macedonia

Stojanovski, Saso and Cilev, Goce and Trajanoska, Blagica (2021) Bacteria variety causing clinical mastitis in Holstein-Friesian cows in Pelagonia region, North Macedonia. AGRICULTURAL SCIENCE AND TECHNOLOGY, 13 (3). pp. 307-312. ISSN 1314-412X

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Abstract. The main aim of this study are the bacteria that most often cause clinical mastitis (CM) and their impact on milk reduction in HolsteinFriesian cows in the Pelagonia - North Macedonia region. 36 milk samples were taken from Holstein-Friesian breed of cows with confirmed
clinical mastitis by a veterinarian. The samples were taken for the period from January 2019 to December 2020 from 20 different smallholder
farms situated in the monitored region. Two sterile tubes with 10 ml of milk in each of them were taken from the affected part of the udder of the
cow. A total of 86 tubes with milk from 36 mastitis cows were taken. From each sample 300 µl drips were placed in petri dishes with different
selective nutrient media: Mannitol Salt Agar, MacConkey Agar, Endo Agar and Edwards nutrient medium. The petri dishes were incubated at
35±2°C for 24-48 hours in Mannitol Salt Agar, at 30-35°C for 18 to 72 hours in MacConkey Agar, at 35±2°C for 18 to 24 hours in Endo Agar
and at 35-37°C for 24-48 hours in Edwards nutrient medium. Morphology of colonies and cells were examined with a microscope. A total of
119 strains were obtained and the following physiological and biochemical studies were performed to determine the new isolates: oxidase
reaction, catalysis activity, indol test, hydrolysis of the hyporate, acetoin formation (acetylmethylcarbinol, Voges-Proscauer reaction) and
Methyl-Roth test (MR- test). The results obtained revealed that the most common bacterial species causing clinical mastitis in Holstein-Friesian
cows in 2019 and 2020 were six species of bacteria, where E. coli and Staphylococcus spp. are dominant.

Item Type: Article
Subjects: Scientific Fields (Frascati) > Agricultural Sciences > Veterinary science
Divisions: Faculty of Veterinary Medicine
Depositing User: Mr Aleksandar Lozanovski
Date Deposited: 29 Oct 2021 09:01
Last Modified: 29 Oct 2021 09:01

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