Tashkoski, Petre (2006) DETECTING THE RESISTANCE OF ORIENTAL TOBACCO VARIETIES TO Phytophtjora parasitica var.nicotianae THROUGH EXCISED LEAF TECHNIQUE. Tobacco, 56 (1-2). pp. 23-30. ISSN 0494-3244

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Variable disease intensity was detected in species and tobacco varieties tested for race O and race 1 of the pathogen, inoculated by the method of whole leaf placed in suspension prepared from pure culture of the fungus. Results of the investigation lead to the following conclusions:
Species N longiflora and N. plumbaginifolia showed total resistance to race O, with no symptoms of infection in the leaves inoculated with mycelia suspension; the same species inoculated with race 1, however, revealed some symptoms of light infection. Disease intensity with this isolate ranged 27,27% in N. longiflora to even 50,00% in N. plumbaginifolia. N. nesophila showed low susceptibility to race 1 (intensity 26,19%), and also to some isolates of race 0 (P 4 and S45), with disease intensity reaching 18,75% and 16,66%, respectively.
The above species confirmed their resistance to race 0 of the pathogen P.parasitica var. nicotianae.
In leaf inoculation with suspension of the fungus mycelia, local varieties showed no significant resistance to races 0 and 1. Difference in pathogenicity of the isolates was noticed here. There were differences in disease intensity between race 0 and race 1 isolates, including also differences among the isolates of race 0 itself. The lowest intensity of disease was noticed in the isolate P4 race 0, ranging from 18,38% in variety B1/91 to 42,58% in P23. The highest disease intensity was noticed in P14 race 0, ranging from 34,48% in variety B1/91 to 65,73% in P23. In other race 0 isolates (P10, S45, P54), the lowest intensity of the disease was recorded in variety B1/91 (24,13%, 24,13% and 22,41%, respectively), and the highest in variety P23 (49,07%, 50,00% and 49,07%, respectively. The isolates P14 race 1 and P10 race 0 appeared to be the most pathogenic.
According to the results, the most susceptible of all investigated varieties was P23, which showed the highest intensity of the disease, while the variety B1/91 was the most tolerant to the isolates of both races, because it had the lowest disease intensity.

Item Type: Article
Subjects: Scientific Fields (Frascati) > Agricultural Sciences > Agriculture, forestry and fisheries
Divisions: Scientific Tobacco Institute
Depositing User: Ms Biljana Jordanoska
Date Deposited: 03 Nov 2016 20:26
Last Modified: 03 Nov 2016 20:26

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