Toxicity of tobacco cuticular extracts against Myzus persicae (Sulzer)-Laboratory bioassay and field trial

Srbinoska, Marija and Simonovska, B. and Vovk, I. and Krsteska, Vesna (2007) Toxicity of tobacco cuticular extracts against Myzus persicae (Sulzer)-Laboratory bioassay and field trial. Plant Science 44, XLIV (2). pp. 114-117.

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Biological insecticide isolated from tobacco (Nicotiana tabacum L.) plants, specific sucrose esters of low fatty acids, has been found to act as effective, envinronmentally safe pesticide against soft bodied arthropod plant pests. They may be a sound alternative to the more persistent synthetic insecticides for managing the infestation of green peach aphid Myzus persicae (Sulzer) on a large number of crops, particularly on commercial tobacco. We obtained a natural sucrose ester mixture in the concentrated form from the surface of green leaves of tobacco cultivar Yaka 125/3 grown at the Tobacco Institute, Prilep in 2005. The trichome exudates and other cuticular components of technologically mature leaves have been quantitatively extracted by dipping leaves into methylene chloride. The solvent was removed by evaporation at 25 oC, and this was a crude or total cuticular extract. We obtained concentrated extract by partitionining crude extract by n-hexane: 80% methanol water 1:1 (v/v) to separate sugar esters from other less polar matter. A normal phase thin-layer chromatographic (TLC) method combined with off-line mass spectroscopy was developed and applied for the detection and quantitative evaluation of sucrose esters. Sucrose esters were the major compounds in the crude and in enriched extract. The emulsifiable concentrates based on cuticular extracts were compared with insecticide Confidor SL 200 (a.i. imidacloprid) for their effectiveness to control a Myzus persicae (Sulzer) in laboratory bioassay and tobacco field trial. We investigated the influence of varying the sugar esters concentration in water solutions, alone and in combination with humectant on the insecticidal activity against Myzus persicae (Sulzer) on tobacco. Crude extract showed 90.23% mortality, the more concentrated extract 93.50%, the mixture of more concentrated extract with humectant 96.90%, the standard insecticide 99.32%, and the control 10.78% mortality in tobacco field trial.

Item Type: Article
Subjects: Scientific Fields (Frascati) > Agricultural Sciences > Agriculture, forestry and fisheries
Divisions: Scientific Tobacco Institute
Depositing User: Ms Biljana Jordanoska
Date Deposited: 07 Oct 2016 16:10
Last Modified: 07 Oct 2016 16:10

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