Triacylglycerols, lipoproteins and cholesterol in human blood

Pavlova, Valentina (2015) Triacylglycerols, lipoproteins and cholesterol in human blood. In: Proceedings of 4th International Conference and Exhibition on Food Processing & Technology, 10-12 August, 2015, London, UK.

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The simplest lipids constructed from fatty acids are the triacylglycerols, also referred to as triglycerides, fats, or neutral fats.
Most natural fats, such as those in vegetable oils, dairy products, and animal fat, are complex mixtures of simple and mixed
triacylglycerols. Cholesterol is doubtless the most publicized lipid, notorious because of the strong correlation between high
levels of cholesterol in the blood and the incidence of human cardiovascular diseases. Various combinations of lipid and protein

produce particles of different densities, ranging from chylomicrons and very lowdensity lipoproteins (VLDL) to very-high-
density lipoproteins (VHDL).

The aim of this study was to determine values of total lipids (triacylglycerols, cholesterol, lipoproteins) in human blood

obtained from patients from municipality Kichevo, Republic of Macedonia. The biochemical analyses were done in P.Z.U. Era-
Medika“Kichevo for 52 patients with different gender and age.

Spectrophotometric method combined with activity of enzymes: lipoproteinlipase, peroxidase, and glycerol kinase was
used for determination of triacylglycerols in human blood. The analysis of cholesterol was performed using enzyme colorimetric
test with Lipid Clearing Factor, and the analysis of HDL-High Density Lipoprotein and LDL-Low Density Lipoprotein was done
using Cholesterol liquicolor test kit, both followed by measuring of absorbance at 500 nm. Unregulated cholesterol production
can lead to serious human disease. When the sum of cholesterol synthesized and cholesterol obtained in the diet exceeds the
amount required for the synthesis of membranes, bile salts, and steroids, pathological
Accumulations of cholesterol in blood vessels (atherosclerotic plaques) can develop, resulting in obstruction of blood
vessels (atherosclerosis). Atherosclerosis is linked to high levels of cholesterol in the blood, and particularly to high levels of
LDL-bound cholesterol; there is a negative correlation between HDL levels and arterial disease. To have well healthy it is very
important to reduce the level of LDL. This can be done by less consumption of food rich with cholesterol and saturated fatty
acids such as eggs, meat products, cheese, butter, ham, full fat milk, coconut and palm oil.

Item Type: Conference or Workshop Item (Poster)
Subjects: Scientific Fields (Frascati) > Natural sciences > Biological sciences
Scientific Fields (Frascati) > Natural sciences > Chemical sciences
Divisions: Faculty of Technology and Technical Sciences
Depositing User: Mr Jordan Martinovski
Date Deposited: 07 Feb 2019 09:32
Last Modified: 07 Feb 2019 09:32

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