Differences between microbiota, phytochemical, antioxidant profil and DNA fingerprinting of cabernet sauvignon grape from Slovakia and Macedonia

Trajkovska Petkoska, Anka (2020) Differences between microbiota, phytochemical, antioxidant profil and DNA fingerprinting of cabernet sauvignon grape from Slovakia and Macedonia. Potravinarstvo Slovak Journal of Food Sciences. (In Press)

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Abstract

The aim of the present study was to evaluate the microbiota, phytochemical, antioxidant profile and DNA fingerprinting of Cabernet Sauvignon grapes from Slovakia and R. North Macedonia. There were used two samples of grape berries (one sample from Slovakia and one from Macedonia). Each sample was analyzed in triplicate. The bacteria were cultivated on Plate count agar (PCA), microscopic filamentous fungi were cultivated on Malt extract agar (MEA). MALDI-TOF MS Biotyper mass spectrometry was used for identification of microorganisms (bacteria and yeasts) and microscopic filamentous fungi with manuals. DPPH Method was used to determine of antioxidant activity of grape berries. Phytochemical and antioxidant profiles were evaluated in grape berries samples. Total genomic DNA was extracted from mature grapes by GeneJET Plant Genomic DNA Purification Kit. The number of bacteria was higher in sample of Macedonian grape (4.13 log CFU/g) in comparison to grape from Slovakia as well as the number of yeasts was also higher in Macedonian sample (2.57 log CFU/g). Antioxidant activity of Slovak grape berries was 0.55 mg TEAC/g and of Macedonian grape berries was 0.51 mg TEAC/g. Total polyphenol content was higher in grape from Slovakia (0.81 mg GAE/g) than in grape from Macedonia (0.77 mg GAE/g), while total flavonoid content was 0.57 and 0.17 mg mg QE/g in Slovak grape and Macedonian grape, respectively. Total phenolic acid content was higher in sample from Macedonia (0.40 mg CAE/g) compared to grape from Slovakia (0.24 mg CAE/g). Total anthocyanin content was also higher in Macedonian grape (0.46 mg/g) compared to Slovak sample (0.05 mg/g). The total polymorphism for all of the used primers of 87.5 % was obtained for the Macedonian sample of Cabernet-Savignon and 89.4 % for Slovak sample.

Item Type: Article
Subjects: Scientific Fields (Frascati) > Agricultural Sciences > Agricultural biotechnology
Divisions: Faculty of Technology and Technical Sciences
Depositing User: Prof. d-r. Anka Trajkovska
Date Deposited: 24 Mar 2020 11:54
Last Modified: 24 Mar 2020 11:54
URI: http://eprints.uklo.edu.mk/id/eprint/4149

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